Journal: Neuron

Article Title: Excitation creates a distributed pattern of cortical suppression due to varied recurrent input.

doi: 10.1016/j.neuron.2023.09.010

Figure Lengend Snippet: Figure 4. Widefield imaging of excitatory neurons shows average center-surround organization during steady-state periods (A) Experimental setup: stChrimsonR in excitatory neurons via viral transfection (AAV-CamKIIa-stChrimsonR), expression of GCaMP via mouse line (either Ai148::Cux2-creERT2, GCaMP6f, or Ai162::Cux2-creERT2, GCaMP6s; induced with tamoxifen as adult; STAR Methods). Right: imaging field of view for one animal. (B and C) Mean deconvolved response (see Figure S3) during first frame (B) and during the late stimulation period (C) in an example animal (fiber for light delivery slightly obstructs the imaging field, see Figures S4D–S4F). (D) Average response over time (n = 3). Red-shaded region: stimulation period, orange bar: first frame, maroon bar: late stimulation (steady state) time period. (E and F) Average responses, n = 3 animals. Responses for each animal were aligned spatially to the peak during the late stimulation period (STAR Methods), smoothed for visualization. (G) Response as a function of distance, averaged from data in (E) and (F). Smoothing: LOWESS. Shaded regions: bootstrapped 95% CIs. Vertical lines: zero crossings and inflection points. Zero crossings defined by shortest distance at which 95% CI included zero. Black lines: late stimulation period. Solid black: first zero crossing, dotted black: local minimum. Red dashed line: early response, first zero crossing. (H and I) Same as (E) and (F) but with superimposed circles whose radii correspond to lines in (G). See also Figures S3 and S4.

Article Snippet: For Ai148 and 162 animals, AAV9-CamKIIa-stChrimsonR-mRuby2 was generated by cloning the CaMKIIa promoter (RRID: Addgene_12021949) into a pAAV backbone containing stChrimsonR-mRuby2 (RRID:Addgene_10544748) and packaged into an AAV (Vigene, Inc.).

Techniques: Imaging, Transfection, Expressing